InCellSX Practical Workshop

16 May 2022, 09:00 → 17 May 2022, 19:15 Europe/Berlin

Title

Approaches for in cellulo structural biology with X-rays

Overview

The advent of high-brilliance synchrotron sources, X-ray free-electron lasers, and improved serial data collection strategies has opened a broad range of new scientific opportunities such as time-resolved serial crystallography, including the use of micro-crystals grown within living cells. The workshop will provide practical on-site training on these research topics and technical approaches. The training will be carried out at relevant infrastructures operated by the European Molecular Biology Laboratory (EMBL) on the DESY Hamburg-Bahrenfeld research campus and by the European X-ray Free Electron Laser in Schenefeld, near Hamburg. Topics for training will be:

• Eukaryotic cell systems for applications in structural biology
• Intracellular protein folding and crystallization
• Detection and characterization of protein crystals
• Fluorescence-activated cell sorting (FACS) techniques for applications in structural biology
• Present and future opportunities in advanced X-ray crystallography
• Sample delivery techniques for X-ray data experiments

 

In addition, there will be lectures by leading invited speakers in the field who will present a comprehensive overview on these topics.

The workshop is primarily targeted to graduate students and postdoctoral fellows working on relevant projects in the field. The registration fee for the workshop is 100 €. Affordable accommodation at local guest houses will be available upon request.

Registration deadline: 10.04.2022 (extended deadline)

Applicants are requested to submit a letter of motivation, a support letter by the supervisor, and their CV. Workshop participants will be selected based on the quality and thematic fit of the application. Decision letters to all applicants will be sent out by the workshop organisers middle of April 2022. We are planning to have the workshop by on-site presence of all workshop participants. In case, the format needs to be changed because of pandemic restrictions, all selected participants will be informed as soon as possible.

The workshop is supported by the Röntgen-Ångström Cluster  project ‘InCellSX – Expanding the domain of serial crystallography: membrane protein & in cellulo crystallization’

This workshop is jointly organized by the European Molecular Biology Laboratory, the University of Lübeck, the European XFEL and the Hanseatic League of Science (HALOS). HALOS  is financed by the Interreg ÖKS Interreg program and aims to strengthen the collaboration between Hamburg and South-West Scandinavia, bring together the four unique research facilities MAX IV, ESS, DESY and European XFEL, and create a centre for integrated, world-leading Life Science innovation and research.

Venue of the workshop:

EMBL Hamburg, Notkestraße 85, 22607 Hamburg, Germany,

and European XFEL GmbH, Holzkoppel 4, 22869 Schenefeld, Germany

Registration

Please submit your CV (pdf.), a short motivation letter (pdf.), and a brief recommendation from your supervisor (pdf.).

Speakers

Confirmed speakers and tutors:

• Isabel Bento, EMBL Hamburg
• Inaki de Diego, European XFEL
• Johanna Hakanpää, DESY, Hamburg
• Haruki Hasegawa, Amgen, South San Francisco
• Adrian Mancuso, European XFEL, Schenefeld
• Alke Meents, DESY, Hamburg
• Pedram Mehrabi, University of Hamburg
• Malte Paulsen, University of Copenhagen
• Lars Redecke, University of Lübeck and DESY, Hamburg
• Kim Remans, EMBL, Heidelberg
• Ekaterina Round, European XFEL
• Thomas Schneider, EMBL Hamburg
• Roland Thünauer, Leibnitz Institute for Experimental Virology, Hamburg
• Raphael de Wijn, European XFEL

Organizers

• Jan Blaha, EMBL, Hamburg
• Lars Redecke, University of Lübeck and DESY, Hamburg
• Ekaterina Round, European XFEL, Schenefeld
• Sihyun Sung, EMBL, Hamburg
• Matthias Wilmanns, EMBL, Hamburg

 

Administrative Support

In case of a questions, concerning your application, please get in touch with Vanya Opalchenska at vanya.opalchenska@email-hamburg.de

Monday, 16 May

09:00 → 09:10 Welcome notes

Speaker: Matthias Wilmanns (EMBL Hamburg)

09:10 → 09:40 Tour de table

Each participant presents his/her work/project in 2 minutes

Speaker: All participants

09:40 → 10:20 Eukaryotic cell systems for applications in structural biology

Speaker: Kim Remans (EMBL Heidelberg)

10:20 → 11:00 In cellulo structural biology: technologies and applications

Speaker: Lars Redecke (University of Lübeck/DESY)

11:00 → 11:20 Break

11:20 → 12:00 Facilities and opportunities for X-ray based structural biology at PETRA III

Speaker: Isabel Bento (EMBL Hamburg)

12:00 → 12:40 Technologies for time-resolved and in cellulo X-ray crystallography experiments

Speaker: Alke Meents (DESY)

12:40 → 13:30 Lunch

13:30 → 17:40 Imaging for detection and characterization for applications of in cellulo structural biology

Practical course

Convener: Roland Thünauer (HPI)

13:30 Group 3

14:40 Break

15:00 Group 1

16:10 Break

16:30 Group 2

13:30 → 17:40 Serial X-ray Crystallography at the P14 beamline T-REXX instrument at PETRA III

Practical course

Convener: Pedram Mehrabi (University of Hamburg)

13:30 Group 1

14:40 Break

15:00 Group 2

16:10 Break

16:30 Group 3

13:30 → 17:40 X-ray data collection, processing & structure determination, using fix target technology

Practical course

Convener: Alke Meents (DESY)

13:30 Group 2

14:40 Break

15:00 Group 3

16:10 Break

16:30 Group 1

17:40 → 18:00 Break

18:00 → 18:45 In cellulo protein folding and crystallization

Speaker: Haruki Hasegawa (Amgen)

19:00 → 20:30 Dinner

Tuesday, 17 May

09:00 → 09:40 FACS techniques for applications of in cellulo structural biology

Speaker: Malte Paulsen (University of Copenhagen)

09:40 → 10:20 Time-resolved X-ray based structural biology

Speaker: Pedram Mehrabi (University of Hamburg)

10:20 → 10:40 Break

10:40 → 11:20 Structural biology using XFEL radiation and instruments

Speaker: Adrian Mancuso (European XFEL)

11:20 → 12:00 What is next in X-ray based structural biology?

Speaker: Thomas Schneider (EMBL Hamburg)

12:00 → 13:30 Lunch and bus transfer to the European XFEL

13:30 → 17:40 Detection and characterization of in cellulo crystals

Practical course

Convener: Ekaterina Round (European XFEL)

13:30 Group 3

14:40 Break

15:00 Group 1

16:10 Break

16:30 Group 2

13:30 → 17:40 FACS for crystals containing cells selection

Practical course

Convener: Malte Paulsen (University of Copenhagen)

13:30 Grou 2

14:40 Break

15:00 Group 3

16:10 Break

16:30 Group 1

13:30 → 17:40 Structural Biology experiments at the SPB/SFX instrument at the European XFEL

Practical course

Convener: Adrian Mancuso (European XFEL)

13:30 Group 1

14:40 Break

15:00 Group 2

16:10 Break

16:30 Group 3

17:40 → 18:00 Break

18:00 → 19:00 Wrap-up session

Speaker: All participants

19:00 → 19:15 Closure notes

Speaker: Lars Redecke (University of Lübeck/DESY)